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AmplifX

Test, manage and design your primers for PCR.
The main purpose of AmplifX is to search through a collection of primers, such as any molecular biologist has in his refrigerators, to find those which can be use to amplify a fragment into a target sequence, for example, and particularly, to design strategies to screen recombinant clones by PCR. Some information is associated with each primer; some automaticelly computed by AmplifX (like TM, Quality, length) and others given by the user (name, comments,...) This allows general aspects of primer management (sequences and real tubes). AmplifX can also design new primers.
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Last update

10/28/08: AmplifX 1.5.4
-  Minor correction of version 1.5.3: calculation of amplicon size was distorded by previous correction of reverse primer position (thanks one more time to Savio Chan, current champion in bug detection!)

10/20/08: AmplifX 1.5.3
-  Cosmetic: suppression of text overlapping in some alert dialogs
-  New option “exclude lowercases” in the primer design’s window (yet implemented in version 1.5.2 but not explained!). This option allows for example to find primers that don’t match against lowercase typed nucleotides in the target sequence. It’s especially useful, for example, to make specific primers for one sequence in a family (type non conserved bases in uppercases) or in the contrary for primers matching conserved regions (type conserved nucleotides in uppercases).
-  Redefining the target sequence origin can now be done by a right-click onto the target sequence after inserting the cursor at the desired position.
-  Primer design: correction of the calculation of the reverse primer position in the result’s list (bug reported by C. Savio Chan)
-  Primer design: adding information to the primers when added to the current primer list: target name, matching position, design date, …) (Feature asked by C. Savio Chan.)
-  Preference window: correction of min and max cutoff scores for weak and strong matches. Sorry this bug appeared in the previous version because of an anticipation of a next major rewriting of the virtual PCR algorithm. (Thanks one more time to C. Savio Chan to saw it.)
-  Search function: correction of some bugs in the “search all occurrences” option.

See the Complete version history

Download english versions :

MAC OS XWindows (98 and above)

Zip - 3.5 Mb
AmplifX 1.5.4 MAC EN

Zip - 1.6 Mb
AmplifX 1.5.4 WIN EN

Help :

-  Quick help.

Awards and citations

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Legal information :

This program is provided free for the scientific community ”as it is". The author will not be held responsible for any bad working.

Users are strongly invited to register themselves by using the menu "feedback" in the "Help" section: give the program name, version number, preferred language, Operating system version, any other useful information and why not a kind word.